NOVA – Tissue and Mammalian Cell Culture RNA Extraction Kit
NOVA Total RNA Purification Kit is designed for rapid and efficient purification of high-quality Total RNA from various mammalian cell cultures and tissue samples, whole blood, bacteria and yeast. This is an innovative mini kit using silica-based membrane technology with an easy and quick to use spin column. This technology eliminates lengthy and complex procedures like the traditional alcohol precipitation or expensive resins methods. The standard procedure takes around 20 minutes following cell lysis. The process yields purified RNA of more than 33kb. Once used, the extracted RNA can be used in RT-PCR, Southern blotting, enzymatic reactions and RNA-sequencing.
For further information, please see below or contact us at info@willowfort.co.uk
Features
- Universal — can be used for cell and tissue samples from a wide range of sources
- Efficient — high yields of high molecular weight RNA
- Fast — this protocol takes only 12-minutes following cell lysis
- Convenient — spin columns are capped and assembled with collection tubes
- Versatile – the RNA product can be used in many downstream applications such as: PCR, qPCR, and Southern Blotting
For research use only. These products should not be used for diagnostics
Use the tables below as a quick guide on how to use the NOVA Tissue and Culture Cells RNA Extraction kit product:
Table 1: Sample weight or Cell counts with expected RNA yields
Tissue type |
Weight or amount |
RNA yield |
Mouse Tail |
0.5cm |
10-20µg |
Mammalian Tissue |
25mg |
10-30µg |
Cultured cells |
1×107 |
10-30µg |
Yeast cells |
2×108 |
10-30µg |
Table 2: Reagents required per sample for extraction
Reagents |
Volume |
Sample amount |
See table 1 |
TRH buffer |
400μl |
70% Ethanol |
400μl |
RW1 buffer |
400μl |
RW2 buffer |
600μl |
Nuclease-free water |
50μl |
Table 3: Reaction times for each step of RNA extraction
Extraction Stage |
Timings |
Sample Lysis |
5 minutes |
RNA Binding |
30-60 seconds |
Washing 1 |
1 minute |
Washing 2 |
1 minute |
Elution |
4 minutes |
For more information about the NOVA Tissue and Cell Culture RNA Extraction kit protocol, please click here for the guide.
To place an order, please contact us through the “Contact Us” link, quoting the Catalog No.
Product |
Package Size | Catalog No. |
NOVA Tissue and Mammalian Cell Culture RNA Extraction Kit | 50 reactions |
WF10104001 |
250 reactions |
WF10104002 |
Storage Conditions
Upon delivery of the NOVA Tissue and Mammalian Cell Culture RNA Extraction kit, store all reagents at room temperature.
Troubleshooting
Please click here for the RNA Extraction Troubleshooting guide.
If you require any additional information about this product please email us at info@willowfort.co.uk