The HERA SYBR® Green qPCR master mix is ready-to-use real-time PCR kit optimised for the real-time quantification of target DNA. In addition to our standard HERA qPCR, the HERA SYBR® Green qPCR master mix includes the SYBR® Green intercalating dye, which binds to double-stranded DNA and releases fluorescence during each reaction cycle, allowing for the genotype of the sample to be quantified.

Utilising our innovative technology, the HERA kit is designed for accurate, highly sensitive and specific qPCR reactions within a wide range of research applications. From gene expression analysis and population genotyping studies to the multiplex detection of several disease targets, SYBR® Green qPCR provides a convenient and powerful method for DNA detection and quantification.

The one-step approach to SYBR® Green qPCR allows for the full reaction to occur in one tube, limiting the potential for contamination and improving reproducibility through reduced sample handling. The HERA SYBR® Green qPCR kit is formulated with a unique passive reference dye that is compatible across a variety of instrument platforms, including those that require a no, low or high ROX reference signal.

For further information, please see below or contact us at info@willowfort.co.uk


  • Efficiency – Using SYBR® Green in the master mix allows the detection of the amplicons with high sensitivity and guarantees the linearity of the qPCR.
  • Accuracy –We ensure all of our products are designed to have superior robustness and accuracy. Additionally, qPCR can be performed using lower amplification times.
  • High yield – Our ready to use master mix ensures HERA Hot Start Taq Polymerase performs at its best to maximise DNA yield.
  • Robust – High fidelity amplification with little optimisation required.

For research use only. These products should not be used for diagnostics.

SYBR is a trademark of Molecular Probes Inc. ROX is a trademark of Life Technologies Corporation

Use the tables below as a quick guide on how to use the HERA SYBR® Green qPCR kit product:

Table 1: Reagents required per sample



HERA SYBR® Green Master Mix (2x)


Forward primers 20x (200nM)

1μl (up to 1μM each)

Reverse primers 20x (200nM)

1μl (up to 1μM each)

Nuclease-free water

To 20μl

DNA template (up to 250ng)

Volume varies


Table 2:  Temperature and Times for the PCR protocol




Stage 1


2 min

Stage 2.1



Stage 2.2



Repeat stage 2 for 40 cycles

*Increase to 1min, if GC content is higher than ≤50%


For more information about the HERA SYBR® Green qPCR kit protocol, please click here for the guide.

To place an order, please contact us through the “Contact Us” link, quoting the Catalog No. and no, low or high ROX.


Package Size Catalog No.

HERA SYBR® Green Green qPCR Kit

100 reactions


500 reactions


1000 reactions


Storage Conditions

Upon delivery of the HERA SYBR® Green qPCR Kit, store all reagents in -20°C.

Troubleshooting Guide

Please click here for the qPCR Troubleshooting guide.

If you require any additional information about this product please email us at info@willowfort.co.uk