COSMOHot Start’ DNA Polymerase Enzyme

COSMO Taq DNA Polymerase is a thermostable DNA polymerase that catalyses the polymerization of nucleotides into duplex DNA in the 5′ -> 3′ direction. COSMO Taq DNA Polymerase is isolated from recombinant E.coli containing the DNA polymerase gene from Thermus aquaticus Y-T1. It exhibits its highest activity at pH 9.0 and 72°C. We recommend using this product in conjunction with COSMO dNTPs (WF1020600X).

The COSMO ‘Hot Start’ DNA Polymerase Enzyme has been reversibly bound by a temperature-dependent inhibitor and so requires treatment at 95°C to become free of this inhibitor. This prevents activation at lower temperatures thus avoiding non-specific amplification of DNA and improving product yields.

For further information, please see below or contact us at


  • Accuracy – the enzyme solution contains antibodies to maximise yield, ensuring that the enzyme is only activated during the elongation phase of the reaction.
  • Reliability – our kits undergo extensive tests to ensure consistent quality in all of our products.
  • Versatility – this ‘Hot Start’ DNA Polymerase Enzyme has a wide array of applications such as Real-Time PCR. It is also compatible with TaqMan® probes and SYBR® Green dyes.
  • High yield and sensitivity – our RED buffer enhances the performance for even the most complex DNA templates, by amplifying them at high fidelity.

For research use only. These products should not be used for diagnostics.

Taqman® is a registered trademark of Roche Molecular Systems, Inc. SYBR® is a registered trademark of Life Technologies Corporation.

Use the tables below as a suggested guide on how to use the COSMO ‘Hot Start’ DNA Polymerase Enzyme product:

Table 1: Reagents required for one reaction



COSMO RED 5x Buffer


COSMO dNTPs mix (not included. Cat no: WF1020600X)


Forward and Reverse primers (0.1—1 μM each)

1-2.5 μl

Nuclease-free water

Up to 50 μl

COSMO ‘Hot Start’ Taq DNA polymerase

5 μl

DNA template (10 pg-1 μg)

 Volume varies


Table 2: Thermal Profile for COSMO ‘Hot Start’ DNA Polymerase Enzyme




Stage 1



Stage 2.1



Stage 2.2

Primer Tm <5oC


Stage 2.3



Repeat stage 2 for 45 cycles

Stage 3


#1 min

*If GC content is <50%, 10 mins is recommended.

** If GC content is <50%, increases this step to 4 mins.

# increase time to 10 mins to maximise performance


For more information about the COSMO ‘Hot Start’ DNA Polymerase Enzyme protocol, please click here for the guide.

To place an order, please contact us through the “Contact Us” link, quoting the Catalog No.


Package Size Catalog No.

COSMO ‘Hot Start’ DNA Polymerase Enzyme


100 Units

500 Units


Storage Conditions

Upon delivery of the COSMO ‘Hot Start’ DNA Polymerase Enzyme, store all reagents in -20°C.

Downstream applications

  • DNA amplification using PCR
  • Genotyping using (Real-Time) qPCR
  • Southern Blotting DNA sequencing
  • DNA sequencing

Troubleshooting Guide

Please click here for the PCR Troubleshooting guide.

If you require any additional information about this product please email us at