NOVA Blood DNA Extraction Kit

NOVA Blood DNA Extraction Kit is an innovative mini kit using silica-based membrane technology with an easy and quick to use spin column. The kit can be used to extract DNA from both the patient and any viruses in the blood, with a high degree of purity, and minimal degradation. This technology eliminates lengthy and complex procedures like alcohol precipitation or expensive resins. The standard procedure takes around 20 minutes following cell lysis. The process yields purified DNA of more than 27kb and can be used, once isolated, in PCR, Southern blotting and enzymatic reactions.

For further information, please see below or contact us at info@willowfort.co.uk

Features

  • High yield– the automation in the extraction procedure minimises product loss.
  • Versatile– the DNA product can be used in many downstream applications such as PCR, qPCR, and Southern Blotting.
  • High purity– our product allows you to exclude enzyme inhibitors, RNA and proteins from the samples to easily transition to your downstream applications

For research use only. These products should not be used for diagnostics

Use the tables below as a quick guide on how to use the NOVA Blood DNA Extraction kit product:

 Table 1: Volumes required for one blood sample extraction

Reagents

Volume

Blood sample

Up to 300μl

BTC buffer

X3 of blood sample volume  + 100μl after pelleting

GLB buffer

200μl

100% Ethanol

200μl

DW1 buffer

400μl

DW2 buffer

600μl

Elution buffer (pH8)

30-100 μl

 

Table 2: Reaction times for each step of DNA extraction

Extraction Stage

Timings

Cell Lysis

20 minutes

DNA Binding

1 minute

Washing 1

1 minute

Washing 2

1 minute

Elution

5 minutes

 

For more information about the NOVA Blood DNA Extraction kit protocol, please click here for the guide.

To place an order, please contact us through the “Contact Us” link, quoting the Catalog No.

Product

Package Size Catalog No.
NOVA Blood DNA Extraction Kit

50 reactions

WF10101001

250 reactions

WF10101002

Storage Conditions

Upon delivery of the NOVA Blood DNA Extraction kit, store all reagents at room temperature.

Troubleshooting

Please click here for the DNA Extraction Troubleshooting guide.

If you require any additional information about this product please email us at info@willowfort.co.uk