NOVA Blood DNA Extraction Kit
NOVA Blood DNA Extraction Kit is an innovative mini kit using silica-based membrane technology with an easy and quick to use spin column. The kit can be used to extract DNA from both the patient and any viruses in the blood, with a high degree of purity, and minimal degradation. This technology eliminates lengthy and complex procedures like alcohol precipitation or expensive resins. The standard procedure takes around 20 minutes following cell lysis. The process yields purified DNA of more than 27kb and can be used, once isolated, in PCR, Southern blotting and enzymatic reactions.
For further information, please see below or contact us at info@willowfort.co.uk
Features
- High yield– the automation in the extraction procedure minimises product loss.
- Versatile– the DNA product can be used in many downstream applications such as PCR, qPCR, and Southern Blotting.
- High purity– our product allows you to exclude enzyme inhibitors, RNA and proteins from the samples to easily transition to your downstream applications
For research use only. These products should not be used for diagnostics
Use the tables below as a quick guide on how to use the NOVA Blood DNA Extraction kit product:
Table 1: Volumes required for one blood sample extraction
Reagents |
Volume |
Blood sample |
Up to 300μl |
BTC buffer |
X3 of blood sample volume + 100μl after pelleting |
GLB buffer |
200μl |
100% Ethanol |
200μl |
DW1 buffer |
400μl |
DW2 buffer |
600μl |
Elution buffer (pH8) |
30-100 μl |
Table 2: Reaction times for each step of DNA extraction
Extraction Stage |
Timings |
Cell Lysis |
20 minutes |
DNA Binding |
1 minute |
Washing 1 |
1 minute |
Washing 2 |
1 minute |
Elution |
5 minutes |
For more information about the NOVA Blood DNA Extraction kit protocol, please click here for the guide.
To place an order, please contact us through the “Contact Us” link, quoting the Catalog No.
Product |
Package Size | Catalog No. |
NOVA Blood DNA Extraction Kit |
50 reactions |
WF10101001 |
250 reactions |
WF10101002 |
Storage Conditions
Upon delivery of the NOVA Blood DNA Extraction kit, store all reagents at room temperature.
Troubleshooting
Please click here for the DNA Extraction Troubleshooting guide.
If you require any additional information about this product please email us at info@willowfort.co.uk