COSMO cDNA Synthesis Kit

COSMO cDNA Synthesis Kit uses the latest developments in reverse transcriptase technology and buffer chemistry to enhance the speed and yield of cDNA synthesis, with accurate transcript representation. The reverse transcriptase, buffer system and combination of random hexamers with anchored oligo (dT) allow for unbiased, efficient and sensitive cDNA synthesis.

High-quality cDNA synthesis for downstream qPCR analysis is essential for successful expression studies. Many factors affect cDNA synthesis including the reverse transcriptase, buffer systems, enhancers and priming strategy. The COSMO cDNA synthesis mix removes the need for user optimisation of these critical factors.

The modified MMLV reverse transcriptase (RTase) is both thermostable and extremely active. The enzyme is blended with RNAase inhibitor preventing degradation of RNA by RNAase contamination, increasing cDNA yield. In addition to increased enzyme kinetics, the RTase is not inhibited by ribosomal and transfer RNAs.

The COSMO cDNA Synthesis kit can be used with 4.0pg to 0.4μg total RNA or oligo(dT) purified mRNA and the relative concentrations of random hexamers and anchored oligo(dT) have been optimised for the generation of cDNA in a fast reliable time. The cDNA product produced from this kit can be used in a variety of downstream applications e.g. real-time PCR or quantitative PCR experiments.

For further information, please see below or contact us at info@willowfort.co.uk

Features

  • Accuracy   The random hexamers with oligo(dT) primers anneal to the RNA aiding in the process of reverse transcription.
  • High yield – At Willowfort,  continued innovations in buffer chemistry and enzymology have drastically improved the performance of the reverse transcriptase.
  • Versatility   The cDNA product can be used in gene cloning, qPCR, and genomic analysis  (e.g. Southern blotting).

For research use only. These products should not be used for diagnostics.

 

Use the tables below as a quick guide on how to use the COSMO cDNA Synthesis Kit product:

Table 1: Reagents per reaction tube

cDNA Reaction Buffer (5x) 4µl
Nuclease-free water Up to 20 µl
RT Enzyme Mix 1 µl
Template RNA (4.0pg-400ng) Volume varies

Annealing at 250C for 5 minutes (up to 10 minutes optional)

Table 2: Recommended Thermal Profile for cDNA Synthesis Kit

cDNA  Step 45* to 500C 15 minutes
Inactivation Step (recommended) 800C to 85*0C

* Optimal Temperature

5 minutes

 

For more information about the COSMO cDNA Synthesis Kit protocol, please click here for the COSMO cDNA Synthesis Kit guide.

 

To place an order, please contact us through the “Contact Us” link, quoting the Catalog No.

Product

Unit Size Catalog No.
COSMO cDNA Kit

20 reactions

WF10205001

100 reactions

WF10205002

Storage Conditions

Upon delivery of the COSMO cDNA Synthesis Kit, store all reagents in -20°C.

Downstream applications

  • DNA amplification using PCR
  • Genotyping using (Real-Time) qPCR
  • Southern Blotting DNA sequencing
  • DNA sequencing

Troubleshooting Guide

Please click here for the PCR Troubleshooting guide.

If you require any additional information about this product please email us at info@willowfort.co.uk