COSMO ‘Hot Start’ PCR Master Mix

The COSMO ‘Hot Start’ PCR Master Mix contains the COSMO ‘Hot Start’ PCR Master Mix, which has been isolated from a recombinant E. coli strain containing the DNA polymerase gene from Thermus aquaticus Y-T1. The Master Mix can be used with DNA templates of up to 5 kb, making it suitable for robust amplifications. This mix contains all the necessary components required for a successful PCR. With an optimised buffer solution and a high fidelity enzyme, all that is required is your DNA template and gene-specific primers. The COSMO ‘Hot Start’  DNA polymerase ensures that non-specific amplification does not occur during PCR set-up, due to the inclusion of antibodies, which maximises specificity by ensuring that the enzyme is only activated during the elongation phase of the reaction. The reaction mix is already optimised for efficient PCR, generating long products at higher yields, whilst minimising contamination risk and the time taken to prepare a reaction.

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Accuracy – The ‘Hot Start’ DNA polymerase Enzyme is only active during the elongation stage due to the inclusion of specific antibodies within the PCR Master Mix.

Robust – High fidelity amplification with little optimisation required, even with complex DNA templates.

Reliable – Willowfort products undergo extensive tests to ensure consistent quality.

Ready to use – Just add the primers and DNA template to the ready-to-use master mix

For research use only. These products should not be used for diagnostics.

Use the tables below as a quick guide on how to use the COSMO ‘Hot Start’ PCR Master Mix product:


Table 1: Reagents required per reaction



COSMO Hot Start PCR Master Mix


Forward and Reverse Primers

1-2.5μl (0.1-1μM each)

Nuclease-free water

To 50μl

DNA template (10pg—1μg)

Volume varies


Table 2:  Temperature and Times for the PCR protocol




Stage 1


*2 min

Stage 2.1


10 sec

Stage 2.2

Primer Tm <5°C

20 sec

Stage 2.3



Repeat stage 2 for 25-35 cycles

Stage 3


1 min

*Increase to 5min if GC content is higher than ≤50%

# The extension time depends upon the amplicon length


For more information about the COSMO ‘Hot Start’ PCR Master Mix, please click here for the guide.


To place an order, please contact us through the “Contact Us” link, quoting the Catalog No.


Package Size Catalog No.

COSMO PCR Master Mix

100 reactions


500 reactions


1000 reactions


Storage Conditions

Upon delivery of the COSMO ‘Hot Start’ PCR Master Mix, store all reagents in -20°C.

Troubleshooting Guide

Please click here for the PCR Troubleshooting guide.

If you require any additional information about this product please email us at