HERA SYBR®  Green RT-qPCR  Kit

HERA SYBR®  Green RT-qPCR  Master Mix is a ready-to-use solution optimised with our random hexamers with anchored oligo(dT) primers for real-time quantification of target RNA using SYBR®  Green. This one-step RT-qPCR provides a convenient and powerful method for gene expression quantification at increased speed and specificity using the HERA Taq Polymerase in the PCR.

This product includes both the reverse transcription and qPCR steps performed in a single tube. Adopting a one-step approach is ideal when no cDNA storage is necessary for subsequent experiments. The approach minimises the risk of contamination and improves reproducibility by reducing sample handling.

The HERA SYBR® Green RT-qPCR master mix provides efficient and highly accurate results by using SYBR®, which intercalates with the amplicon to generate a fluorescent signal. This product is suitable for many research applications from gene expression analysis to cDNA, population genotyping studies and the detection of disease targets.

For further information, please see below or contact us at info@willowfort.co.uk

Features

  • Accuracy – We ensure all of our probes have high specificity to the target area.
  • Efficiency – Using SYBR green in the master mix allows you to detect the amplicons with highly sensitive and accurate amplification for the target region.
  • Robust – High fidelity amplification with little optimisation required, producing consistent and reliable results.
  • Simple and time efficient –  this user-friendly protocol and convenient master mix, minimises sample handling and time spent preparing each sample.
  • Target flexibility – single or multiplex reactions can be performed
  • High yield – our buffer ensures RT enzyme mix and the DNA Polymerase performs at its best to maximise RNA target yield

For research use only. These products should not be used for diagnostics.

SYBR is a trademark of Molecular Probes Inc. ROX is a trademark of Life Technologies Corporation

Use the tables below as a quick guide on how to use the HERA SYBR® Green RT-PCR Master Mix product:

Table 1: Reagents required per sample

Components

Amount

RT Enzyme Mix (20X)

1μl

HERA SYBR® Green RT-qPCR Master Mix (2x)

10μl

Forward primers 20x (200nM)

1μl (up to 1μM each)

Reverse primers 20x (200nM)

1μl (up to 1μM each)

Nuclease-free water

To 20μl

RNA template (up to 250ng)

Volume varies

 

Table 2:  Temperature and Times for the PCR protocol

Stage

Temperature

Time

Stage 1

Reverse Transcription

50-55°C

15mins

Stage 2

Enz. Activation

95°C

5mins

Stage 3.1

Denaturation

95°C

*10sec

Stage 3.2

Annealing/Extension

60°C

30sec

Repeat stage 3 for 40 cycles

*Increase to 1min, if GC content is ≤50%

 

For more information about the HERA SYBR® Green RT-PCR Master Mix protocol, please click here for the guide.

To place an order, please contact us through the “Contact Us” link, quoting the Catalog No and no, low or high ROX.

Product

Package Size Catalog No.

HERA SYBR® Green RT-qPCR Kit

100 reactions

WF10303001

500 reactions

WF10303002

1000 reactions

WF10303003

Storage Conditions

Upon delivery of the HERA SYBR® Green RT-qPCR Kit, store all reagents in -20°C.

Troubleshooting Guide

Please click here for the RT-qPCR Troubleshooting guide.

If you require any additional information about this product please email us at info@willowfort.co.uk