HERA qPCR Kit with UDG

The HERA qPCR probe master mix with UDG is a ready-to-use real-time PCR kit optimised for the real-time quantitation of target DNA using fluorophore labelled probes. Utilising our innovative technology, the HERA kit is designed for accurate, highly sensitive and specific qPCR reactions. The kit also includes the uracil-DNA-glycosylase (UDG) system, preventing any contamination and amplification of uracil containing amplicons.

The kit is suitable for a wide range of research applications, making the HERA qPCR kit with UDG a convenient and powerful method for DNA detection and quantification.

The one-step approach to qPCR allows for the full reaction to occur in one tube, limiting the potential for contamination and improving reproducibility through reduced sample handling. The ability to detect multiple amplicons in one tube is the principle advantage of this probe-based technology, unlike traditional technologies which are limited by light channels in the real-time PCR instrument.

HERA is formulated with a unique passive reference dye that is compatible across a variety of instrument platforms, including those that require a no, low and high ROX reference signal.

For further information, please see below or contact us at info@willowfort.co.uk.


  • Efficient– Using multiple probes in the master mix allows detection of multiple amplicons (fluorophore signals) in the same tube.
  • Accurate– Our probe master mix shows high specificity during amplification of the target area.
  • High yield– The ready-to-use master mix ensures optimal performance of HERA Taq Polymerase to maximise DNA yield with little optimisation required.
  • Robust– High fidelity amplification with little optimisation required. Amplification for products up to 5 kilobases.
  • Reliable– The UDG system reduces the risk of PCR product contamination in high throughput labs, giving peace of mind.

For research use only. These products should not be used for diagnostics

Use the tables below as a guide on how to use this product:

Table 1: Reagents required per sample



HERA Master Mix with UDG (2X)


Forward Primer 20X (200nM)

1μl (up to 1μM each)

Reverse Primer 20X (200nM)

1μl (up to 1μM each)

Hydrolysis Probe 20X (up to 300nM)


Nuclease-Free Water

To 20μl

Template DNA (up to 250ng)



Table 2:  Temperature and Times for the PCR protocol




Stage 1.1

UDG Activation


5 min

Stage 1.2

HERA Enz. Activation


*2 min

Stage 2.1




Stage 2.2#

Annealing /Extension



Repeat Stage 2 for 40 cycles

* For GC rich (≥50% GC) DNA templates, 5 minutes is recommended.

**For GC rich DNA, increase this step for up to 1 minute

#Data collection step in the probe channel


For more information about the HERA qPCR with UDG kit protocol, please click here for the guide.

To place an order, please contact us through the “Contact Us” link, quoting the Catalog No. and no, low or high ROX.


Package Size Order Information

HERA qPCR Kit with UDG

100 reactions


500 reactions


1000 reactions


Storage Conditions

Upon delivery of the HERA qPCR with UDG Kit, store all reagents in -20°C.

Troubleshooting Guide

Please click here for the qPCR Troubleshooting guide.

If you require any additional information about this product please email us at info@willowfort.co.uk