HERA RT-qPCR Kit

HERA RT-qPCR Kit is a ready-to-use solution optimised for efficient and simple real-time quantification of target RNA using hydrolysis probes. This one-step RT-qPCR provides a convenient and powerful method for RNA detection and quantification at increased speed and specificity. The HERA RT-qPCR Kit provides quick and accurate results and is suitable for many research applications from gene expression analysis using cDNA and population genotyping studies, to the multiplex detection of several disease targets.

In the one-step approach to HERA RT-qPCR kit, both the reverse transcription step and qPCR steps are performed in a single tube. Adopting a one-step approach is ideal when no cDNA storage is necessary for subsequent experiments. The approach minimises the risk of contamination and improves reproducibility by reducing sample handling.

For further information, please see below or contact us at info@willowfort.co.uk.

Features

  • Accurate– we ensure all of our probes have high specificity to the target area
  • Efficient– Using multiple probes in the master mix allows detection of multiple amplicons (fluorophore signals) in the same tube.
  • Robust – High fidelity amplification with little optimisation required, producing consistent and reliable results
  • Simple and time efficient –  this user-friendly protocol and convenient master mix, minimises sample handling and time spent preparing each sample.
  • Target flexibility – single or multiplex reactions can be performed
  • High yield – our buffer ensures RT enzyme mix and the DNA Polymerase performs at its best to maximise RNA target yield

For research use only. These products should not be used for diagnostics.

ROX is a trademark of Life Technologies Corporation

Use the tables below as a quick guide on how to use the HERA RT-PCR kit product:

Table 1: Reagents required per sample

Components

Amount

RT Enzyme Mix (20X)

1μl

HERA RT-qPCR Master Mix (2x)

10μl

Forward primers 20x (200nM)

1μl (up to 1μM each)

Reverse primers 20x (200nM)

1μl (up to 1μM each)

Hydrolysis Probe 20X (up to 300nM)

1μl

 

Nuclease-free water

To 20μl

RNA template (up to 250ng)

Volume varies

 

Table 2:  Temperature and Times for the PCR protocol

Stage

Temperature

Time

Stage 1

Reverse Transcription

50-55°C

15mins

Stage 2

Enz. Activation

95°C

5mins

Stage 3.1

Denaturation

95°C

*10sec

Stage 3.2

Annealing/Extension

60°C

30sec

Repeat stage 3 for 40 cycles

*Increase to 1min, if GC content is ≤50%

 

For more information about the HERA RT-PCR kit protocol, please click here for the guide.

 

To place an order, please contact us through the “Contact Us” link, quoting the Catalog No. and no, low or high ROX.

Product

Package Size Order Information

HERA RT-qPCR Kit

100 reactions

WF10301001

500 reactions

WF10301002

1000 reactions

WF10301003

Storage Conditions

Upon delivery of the HERA RT-qPCR Kit, store all reagents in -20°C.

Troubleshooting Guide

Please click here for the RT-qPCR Troubleshooting guide.

If you require any additional information about this product please email us at info@willowfort.co.uk