COSMO ‘Hot Start’ PCR RED Master Mix
The COSMO ‘Hot Start’ PCR RED Master Mix is a ready-to-use solution that enables robust amplification up to 5 kb of genetic material from original template DNA. This mix contains all the necessary components required for a successful PCR, as well as red loading dye to visualise pipetting steps and to aid agarose gel loading. This all-in-one product improves time-efficiency and reduces the chance of external contamination post-PCR. All that needs adding are the DNA template and gene-specific primers. The ‘Hot Start’ Taq polymerase is isolated from a recombinant E. coli strain containing the DNA polymerase gene from Thermus aquaticus Y-T1. The enzyme has been modified to eliminate non-specific amplification during PCR set-up by the addition of antibodies within the buffer to maximise specificity. The reaction mix is already optimised for efficient PCR, generating long products at higher yields whilst minimising contamination risk and the time taken to prepare a reaction.
For further information, please see below or contact us at info@willowfort.co.uk.
Features
- Accuracy – the COSMO RED buffer provides the optimal pH and Mg2+ to enhance enzyme performance, producing high yield PCR products.
- Convenient – the COSMO RED buffer contains gel loading dye to aid gel loading, removing this step post-PCR.
- Robust – High fidelity amplification with little optimisation required, even with complex DNA templates.
- Reliable – Willowfort products undergo extensive tests to ensure consistent quality.
- Ready to use – Just add gene-specific primers and your DNA template to the master mix.
For research use only. These products should not be used for diagnostics.
Use the tables below as a quick guide on how to use the COSMO ‘Hot Start’ PCR RED Master Mix product:
Table 1: Reagents required per sample
Components |
Amount |
COSMO ‘Hot Start’ PCR Master Mix RED |
25μl |
Forward and Reverse Primers |
1-2.5μl (0.1-1μM each) |
Nuclease-free water |
To 50μl |
DNA template (10pg—1μg) |
Volume varies |
Table 2: Temperature and Times for the PCR protocol
Stage |
Temperature |
Time |
||
Stage 1 |
95°C |
*2 min |
||
Stage 2.1 |
95°C |
10 sec |
||
Stage 2.2 |
Primer Tm <5°C |
20 sec |
||
Stage 2.3 |
72°C |
#30-60s |
||
Repeat stage 2 for 25-35 cycles |
||||
Stage 3 |
72°C |
1 min |
||
*Increase to 5min if GC content is higher than ≤50% # The extension time depends upon the amplicon length |
For more information about the COSMO ‘Hot Start’ PCR RED Master Mix protocol, please click here for the guide.
To place an order, please contact us through the “Contact Us” link, quoting the Catalog No.
Product |
Package Size | Catalog No. |
COSMO Hot Start PCR Master Mix RED |
100 reactions |
WF10208001 |
500 reactions |
WF10208002 |
|
1000 reactions |
WF10208003 |
Storage Conditions
Upon delivery of the COSMO ‘Hot Start’ PCR RED Master Mix, store all reagents in -20°C.
Troubleshooting Guide
Please click here for the PCR Troubleshooting guide.
If you require any additional information about this product please email us at info@willowfort.co.uk