HERA SYBR® Green qPCR Kit with UDG

The HERA SYBR® Green qPCR master mix with UDG is a ready-to-use real-time PCR kit optimised for the quantitation of target DNA using a double-stranded DNA binding dye. Utilising our innovative technology, the HERA kit is designed for accurate, highly sensitive and specific double-stranded DNA binding dye qPCR reactions.  The kit also includes the uracil-DNA-glycosylase (UDG) carryover prevention system,  ensuring any contaminating, uracil-containing amplicons from previous reactions are not reamplified in the current reaction.

The kit is suitable for a wide range of research applications. From gene expression analysis using cDNA and population genotyping studies to the multiplex detection of several disease targets, qPCR provides a convenient and powerful method for DNA detection and quantitation.

The one-step approach to qPCR allows for the full reaction to occur in one tube, limiting the potential for contamination and improving reproducibility through reduced sample handling.

HERA master mix is formulated with a unique passive reference dye that is compatible across a variety of instrument platforms, including those that require a no, low and high ROX reference signal.

For further information, please see below or contact us at info@willowfort.co.uk.


  • Efficient – Using SYBR® green in the master mix allows the detection of amplicons with high sensitivity and guarantees the linearity of the qPCR.
  • Accurate –The HERA SYBR® Green qPCR kit with UDG is designed to have superior robustness and accuracy.  Additionally, qPCR can be performed using shorter amplification times.
  • High yield – The ready-to-use master mix ensures optimal performance of  HERA Hot Start Taq Polymerase to maximise DNA yield with little optimisation required.
  • Reliable – The UDG system reduces the risk of PCR product contamination in high throughput labs, giving peace of mind.

For research use only. These products should not be used for diagnostics.

Use the  tables below as a guide on how to use this product:

Table 1: Reagents required per sample



HERA SYBR® Master Mix with UDG (2X)


Forward Primer 20X (200nM)

1μl (up to 1mM)

Reverse Primer 20X (200nM)

1μl (up to 1mM)

Nuclease-Free Water

To 20 μl

Template DNA (up to 250ng)



Table 2:  Temperature and Times for the PCR protocol


Temp. Time
Stage 1.1

UDG Activation


5 min

Stage 1.2

HERA Enz. Activation


*2 min

Stage 2.1


95°C **10s
Stage 2.2#

Annealing /Extension



Repeat Stage 2 for 40 cycles

* For GC rich (≥50% GC) DNA templates, 5 minutes is recommended.

**For GC rich DNA, increase this step for up to 1 minute

#Data collection step in the probe channel


For more information about the HERA SYBR® Green qPCR Kit with UDG protocol, please click here for the guide.

To place an order, please contact us through the “Contact Us” link, quoting the Catalog No. and no, low or high ROX.


Package Size Order Information

HERA SYBR® Green qPCR Kit with UDG

100 reactions


500 reactions


1000 reactions


Storage Conditions

Upon delivery of the HERA SYBR® Green qPCR Kit with UDG, store all reagents in -20°C.

Troubleshooting Guide

Please click here for the qPCR Troubleshooting guide.

If you require any additional information about this product please email us at info@willowfort.co.uk