DNA & RNA Synthesis
Oligonucleotide synthesis
Willowfort provides an extensive range of custom oligo synthesis services, from simple PCR primers to highly modified oligonucleotides. All of our products are purified and subject to QC by mass spectrometry. Oligonucleotides are synthesized using Phosphoramidite solid-phase synthesis and are purified by high-performance liquid chromatography (HPLC) or PAGE to achieve the desired purity and are subject to QC by Mass Spectrometry. Synthetic oligonucleotides obtained can be used in a variety of molecular biology applications such as PCR, qPCR, sequencing, mutagenesis, SNP assays, microarrays, etc.
Services:
- Custom DNA and RNA oligo synthesis, including primer/probe synthesis and labelling
- Standard and custom long oligo synthesis
- BNA synthesis
- 2ml screw-top tubes or 96-well plates
- Aliquoting available
- Delivery format: lyophilised or normalised to a selected concentration
DNA Oligo synthesis Primers, labelled or unlabelled Probes, labelled or unlabelled Short or long DNA oligos (up to 300 bases) Wide choice of modifications
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RNA Oligo synthesis
Wide choice of modifications Short or long RNA oligos (up to 300 bases) |
BNA Oligo synthesis Replacement for LNA and PNA Superior binding affinity |
Quality Control System
High quality oligonucleotides require a rigorous quality management system in place. All the reagents used in this process are our own and checked for quality prior to use. We monitor the oligonucleotides during synthesis in order to control the coupling efficiency at each cycle. All oligonucleotides are analysed by HPLC or PAGE for purity and characterized by mass spectrometry. Finally, oligonucleotides are quantitated twice by UV spectrophotometry to provide an accurate measure of yield.
Ordering & Contact Information
Unmodified Oligonucleotide |
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Product | 40 nmol scale | 100 nmol scale | 200 nmol scale | 1 umol scale | 2 umol scale | 10 umol scale | Custom scale |
Catalog No. | £0.4 | £0.6 | £1.1 | £3 | £5.5 | £30 | —— |
Max. Length | 70 mer | 110 mer | 110 mer | 200 mer | 200 mer | 200 mer | 200 mer |
Purification Price Per DNA Oligo |
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Product | 40 nmol scale | 100 nmol scale | 200 nmol scale | 1 umol scale | 2 umol scale | 10 umol scale | Custom scale |
PAGE | – | £80 | £80 | £200 | £300 | £500 | —— |
HPLC | – | £40 | £40 | £120 | £180 | £300 | —— |
- Please contact us for additional information or send an email to oligo@willowfort.co.uk
- To contact us by phone, please call
- Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system
- When using a credit card it will be billed under
Custom DNA oligonucleotide can be ordered by email oligo@willowfort.co.uk
Modification Oligonucleotide
- You can modify your oligonucleotide with numerous fluorophores and quenchers from an extensive list of modifications.
Our modified oligonucleotide of dual-labelled probes. Our dual dyed probes are highly sensitive, dual-labelled DNA probes. We offer a wide variety of quenchers and fluorescent reporter dyes for all types of qPCR platforms
Fluorophore | Absorbance (nm) | Emission (nm) | Scale 50nmol | Scale 100nmol | Scale
200nmol |
Scale 1umol |
FAM™ | 494 | 518 | · | · | · | · |
TET™ | 521 | 536 | · | · | · | · |
JOE™ | 528 | 548 | · | · | ||
HEX™ | 535 | 556 | · | · | · | · |
NED™ | 546 | 575 | · | · | ||
ROX™ | 575 | 602 | · | · | · | |
TAMRA™ | 555 | 580 | · | · | · | · |
TET™ | 521 | 536 | · | · | · | |
Cy 3.5 | 588 | 604 | · | · | ||
Cy 5 | 646 | 667 | · | · | · | |
Most of Quenchers are available suitable for your Fluorophore |