COSMO PCR RED Master Mix
The COSMO PCR RED Master Mix is a ready-to-use solution that can be used for robust amplification. This product can routinely amplify original template DNA of up to 5 kb. The mix contains the COSMO DNA Polymerase Enzyme, as well as all the necessary components required for a successful PCR. All that is left is to add your DNA template and gene-specific primers, saving time when preparing your reactions. The COSMO PCR RED Master Mix is already optimised for efficient PCR reactions and generates long products with a higher yield, whilst minimising contamination risk and the time taken to prepare a reaction.
For further information, please see below or contact us at info@willowfort.co.uk
Features
- Robust – High fidelity amplification with little optimisation required.
- Reliable – Willowfort kits undergo extensive tests to ensure consistent quality in all products.
- High yield and sensitivity – COSMO buffer enhances the performance for even the most complex DNA templates by amplifying them at high fidelity.
- Ready to use – Just add the primers and DNA template to the master mix for an easy to use protocol.
For research use only. These products should not be used for diagnostics.
Use the tables below as a quick guide on how to use the COSMO PCR RED Master Mix product:
Table 1: Reagents required per sample
Reagents |
Volume |
COSMO PCR RED Master Mix |
25μl |
Forward and Reverse Primers (20μM) |
1—2.5μl (0.1—1μM each) |
Nuclease-free water |
To 50μl |
DNA template (10pg—1μg) |
Volume varies |
Table 2: Temperature and Times for the PCR protocol
Stage |
Temperature |
Time |
||
Stage 1 |
95°C |
*2 min |
||
Stage 2.1 |
95°C |
15 sec |
||
Stage 2.2 |
Primer Tm <5°C |
20 sec |
||
Stage 2.3 | 72°C |
#30-60s |
||
Repeat stage 2 for 25-35 cycles |
||||
Stage 3 |
72°C |
1 min |
||
*Increase to 5min if GC content is higher than ≤50% # The extension time depends upon the amplicon length |
For more information about the COSMO PCR Master Mix protocol, please click here for the guide.
To place an order, please contact us through the “Contact Us” link, quoting the Catalog No.
Product |
Package Size | Catalog No. |
COSMO PCR RED Master Mix |
100 reactions |
WF10203001 |
200 reactions |
WF10203002 |
|
1000 reactions |
WF10203003 |
Storage Conditions
Upon delivery of the COSMO PCR RED Master Mix, store all reagents at -20°C.
Troubleshooting Guide
Please click here for the PCR Troubleshooting guide.
If you require any additional information about this product please email us at info@willowfort.co.uk