NOVA Blood RNA Extraction Kit
NOVA Total RNA Purification Kit is designed for rapid and efficient purification of high-quality Total RNA from blood samples. This is an innovative mini kit using silica-based membrane technology with an easy and quick to use spin column. This technology eliminates lengthy and complex procedures like alcohol precipitation or expensive resins. The standard procedure takes around 20 minutes following cell lysis. The process yields purified RNA of more than 33kb and can be used, once isolated, in PCR, northern blotting and enzymatic reactions.
This product allows you to extract RNA with a high degree of purity, and minimal degradation of the RNA product using silica-based membrane technology. This kit can be used to extract RNA from the leukocytes in the blood. This product degrades any DNA from the sample to maximise purity.
For further information, please see below or contact us at info@willowfort.co.uk
Features
- High yield– the automation in the extraction procedure minimises product loss.
- Versatile– the RNA product can be used in many downstream applications such as RT-PCR, RT-qPCR, and Southern Blotting.
- High purity– our allows you to exclude enzyme inhibitors, RNA and proteins from the samples to easily transition to your downstream applications.
For research use only. These products should not be used for diagnostics
Use the tables below as a quick guide on how to use the NOVA Blood RNA Extraction kit product:
Table 1: Volumes required for one blood sample extraction
Reagents |
Volume |
Blood sample |
Up to 300μl |
RBC buffer |
X3 of blood sample volume + 100μl after pelleting |
RLB buffer |
200μl |
100% Ethanol |
200μl |
RW1 buffer |
400μl |
RW2 buffer |
600μl |
Nuclease-free water |
30-100μl |
Table 2: Reaction times for each step of RNA extraction
Extraction Stage |
Timings |
Cell Lysis |
15 minutes |
RNA Binding |
30-60 seconds |
Washing 1 |
30-60 seconds |
Washing 2 |
30-60 seconds |
Elution |
5 minutes |
For more information about the NOVA Blood RNA Extraction kit protocol, please click here for the guide.
To place an order, please contact us through the “Contact Us” link, quoting the Catalog No.
Product |
Package Size |
Catalog No. |
NOVA Blood RNA Extraction Kit |
50 reactions |
WF10102001 |
250 reactions |
WF10102002 |
Storage Conditions
Upon delivery of the NOVA Blood RNA Extraction kit, store all reagents at room temperature.
Troubleshooting
Please click here for the RNA Extraction Troubleshooting guide.
If you require any additional information about this product please email us at info@willowfort.co.uk