NOVA – Tissue and Mammalian Cell Culture RNA Extraction Kit

NOVA Total RNA Purification Kit is designed for rapid and efficient purification of high-quality Total RNA from various mammalian cell cultures and tissue samples, whole blood, bacteria and yeast. This is an innovative mini kit using silica-based membrane technology with an easy and quick to use spin column. This technology eliminates lengthy and complex procedures like the traditional alcohol precipitation or expensive resins methods. The standard procedure takes around 20 minutes following cell lysis. The process yields purified RNA of more than 33kb. Once used, the extracted RNA can be used in RT-PCR, Southern blotting, enzymatic reactions and RNA-sequencing.

For further information, please see below or contact us at info@willowfort.co.uk

Features

  • Universal — can be used for cell and tissue samples from a wide range of sources
  • Efficient — high yields of high molecular weight RNA
  • Fast — this protocol takes only 12-minutes following cell lysis
  • Convenient — spin columns are capped and assembled with collection tubes
  • Versatile – the RNA product can be used in many downstream applications such as: PCR, qPCR, and Southern Blotting

For research use only. These products should not be used for diagnostics

Use the tables below as a quick guide on how to use the NOVA Tissue and Culture Cells RNA Extraction kit product:

 

Table 1: Sample weight or Cell counts with expected RNA yields

Tissue type

Weight or amount

RNA yield

Mouse Tail

0.5cm

10-20µg

Mammalian Tissue

25mg

10-30µg

Cultured cells

1×107

10-30µg

Yeast cells

2×108

10-30µg

 

Table 2: Reagents required per sample for extraction

Reagents

Volume

Sample amount

See table 1

TRH buffer

400μl

70% Ethanol

400μl

RW1 buffer

400μl

RW2 buffer

600μl

Nuclease-free water

50μl

 

Table 3: Reaction times for each step of RNA extraction

Extraction Stage

Timings

Sample Lysis

5 minutes

RNA Binding

30-60 seconds

Washing 1

1 minute

Washing 2

1 minute

Elution

4 minutes

 

For more information about the NOVA Tissue and Cell Culture RNA Extraction kit protocol, please click here for the guide.

To place an order, please contact us through the “Contact Us” link, quoting the Catalog No.

Product

Package Size Catalog No.
NOVA Tissue and Mammalian Cell Culture RNA Extraction Kit 50 reactions

WF10104001

250 reactions

WF10104002

Storage Conditions

Upon delivery of the NOVA Tissue and Mammalian Cell Culture RNA Extraction kit, store all reagents at room temperature.

Troubleshooting

Please click here for the RNA Extraction Troubleshooting guide.

If you require any additional information about this product please email us at info@willowfort.co.uk