HERA qPCR Kit

The HERA qPCR probe master mix is a ready-to-use real-time PCR kit optimised for the quantification of target DNA. Utilising our innovative technology, the HERA qPCR kit is designed for accurate, highly sensitive and specific reactions within a wide range of research applications. From gene expression analysis using cDNA and population genotyping studies to the multiplex detection of several disease targets, qPCR provides a convenient and powerful method for DNA detection and quantification.

The ability to detect multiple amplicons in one tube is the principle advantage of this probe-based technology, unlike traditional technologies which are limited by light channels in the real-time PCR instrument.

HERA is formulated with a unique passive reference dye that is compatible across a variety of instrument platforms, including those that require a no, low or high ROX reference signal.

For further information, please see below or contact us at info@willowfort.co.uk

Features

  • Efficiency – Using multiple probes and the HERA Taq Polymerase in the master mix allows detection of multiple amplicons in the same tube in a time efficient manner.
  • Accuracy – We ensure all of our probes have high specificity to the target area.
  • High yield – The ready-to-use master mix ensures optimal performance of HERA Taq Polymerase to maximise DNA yield.
  • Robust – High fidelity amplification of products up to 5 kilobases with little optimisation required.

For research use only. These products should not be used for diagnostics.

Use the tables below as a quick guide on how to use the HERA qPCR kit product:

Table 1: Reagents required per sample

Components

Amount

HERA Master Mix (2x)

10μl

Forward primers 20x (200nM)

1μl (up to 1μM each)

Reverse primers 20x (200nM)

1μl (up to 1μM each)

Hydrolysis Probe 20x (up to 300nM)

1μl

Nuclease-free water

To 20μl

DNA template (up to 250ng)

Volume varies

 

Table 2:  Temperature and Times for the PCR protocol

Stage

Temperature

Time

Stage 1

95°C

2 min

Stage 2.1

95°C

*10sec

Stage 2.2

60°C

30sec

Repeat stage 2 for 40 cycles

*Increase to 1min, if GC content is higher than ≤50%

 

For more information about the HERA qPCR kit protocol, please click here for the guide.

 

 

To place an order, please contact us through the “Contact Us” link, quoting the Catalog No. and no, low or high ROX.

Product

Package Size Catalog No.

HERA qPCR Kit

100 reactions

WF10302001

500 reactions

WF10302002

1000 reactions

WF10302003

Storage Conditions

Upon delivery of the HERA qPCR Kit, store all reagents in -20°C.

Troubleshooting Guide

Please click here for the qPCR Troubleshooting guide.

If you require any additional information about this product please email us at info@willowfort.co.uk